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Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose (Rosa roxburghii tratt) fruit

Chen, Guangjing, Kan, Jianquan
Journal of food science and technology 2018 v.55 no.3 pp. 1083-1092
2,2-diphenyl-1-picrylhydrazyl, Rosa roxburghii, antioxidant activity, antioxidants, arabinose, blood serum, catalase, cosmetics, drugs, free radicals, functional foods, galactose, galacturonic acid, glucose, glucuronic acid, glutathione peroxidase, liver, malondialdehyde, mannose, mice, molecular weight, polysaccharides, raw materials, response surface methodology, rhamnose, superoxide dismutase, temperature, ultrasonic treatment, ultrasonics, value added, xylose
In this study, the response surface methodology was utilized to determine optimum conditions for extracting the polysaccharides from Rosa roxburghii Tratt fruit (RRTPs) using ultrasonic-assisted extraction, and the characterization and antioxidant activities of the RRTPs were discussed. RRTPs yield was 6.59 ± 1.34%, which was well consistent with the predicted value of 6.716%, under the following optimum conditions: ratio of water to raw material 40.18 mL/g, extraction temperature 78.8 °C, ultrasonic power 148 W, and extraction time 32.8 min. The monosaccharide composition analysis indicated that RRTPs were composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara) and xylose (Xyl). The molecular weight distribution analysis showed that RRTPs had four main components with molecular weights of 332.56, 183.96, 11.92 and 5.95 kDa, respectively. In vitro antioxidant studies revealed RRTPs exhibited significant antioxidant potential on hydroxyl, superoxide and DPPH radicals. In addition, antioxidant assays in vivo demonstrated that RRTPs can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and total antioxidant capacity (TAOC) to some extent, as well as decrease the level of malondialdehyde (MDA) in both serum and liver of D-Gal aging-induced mice. These data suggested that RRTPs could be a potential candidate of natural antioxidants for applications in functional food, pharmaceuticals or cosmetic industries. In summary, this work provided an effective method for the exploitation and utilization of value-added R. roxburghii Tratt fruit which would be useful to fully utilize this resource.