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Ranking filter methods for concentrating pathogens in lake water
- Borchardt, Mark A., Kieke Jr., Burney A., Spencer, Susan K.
- Applied and environmental microbiology 2013 v.79 no.17 pp. 5418
- filtration, lakes, pathogens, quantitative polymerase chain reaction, surface water
- Accurately comparing filtration methods for concentrating waterborne pathogens is difficult because of two important water matrix effects on recovery measurements, the effect on PCR quantification and the effect on filter performance. Regarding the first effect, we show how to create a control water matrix, with characteristics identical to the test water matrix, to avoid unequal error biases between qPCR measurements of the recovered pathogen (the dividend of the percent recovery calculation) and the pathogen seed (the divisor). As for the water matrix effect on filter performance, it can be controlled statistically or removed experimentally by simultaneous side-by-side recovery trials. Cost, ease-of-use, requirements for recovery controls, and the capability to achieve study objectives are additional considerations when selecting a filter method.