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Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases

Author:
Bezsudnova, Ekaterina Yu., Dibrova, Daria V., Nikolaeva, Alena Yu., Rakitina, Tatiana V., Popov, Vladimir O.
Source:
Journal of biotechnology 2018 v.271 pp. 26-28
ISSN:
0168-1656
Subject:
amines, amino acids, bioinformatics, catalytic activity, transaminases
Abstract:
New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)-1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-amine:pyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Sequences encoding new transaminases with mixed type of activity were found by searching for changes in the conserved motifs of sequences of BCAT by different bioinformatics tools.
Agid:
5907661