PubAg

Main content area

Fractionation of milk proteins on pilot scale with particular focus on β-casein

Author:
Thienel, Katharina J.F., Holder, Aline, Schubert, Thomas, Boom, Remko M., Hinrichs, Jörg, Atamer, Zeynep
Source:
International dairy journal 2018 v.79 pp. 73-77
ISSN:
0958-6946
Subject:
beta-casein, fractionation, isolation techniques, kappa-casein
Abstract:
The aim of this study was to increase the yield and purity of casein fractions at pilot scale and determine the main process parameters influencing the isolation of β-casein, such as cold extraction time, separation speed. The fractions were obtained from micellar casein by means of selective precipitation following separation by a nozzle centrifuge. The purities, which were calculated based on the total casein content of the β-casein fraction, and the yields achieved for β-casein ranged from 68.7 to 89.6% and from 10 to 32%, respectively. For the other two fractions, αS- and κ-casein, the obtained purities were 61% and 43%, respectively. Using the proposed isolation method, high purities for the β-casein fraction were achievable; however, the other two fractions, αS- and κ-casein, need further improvement.
Agid:
5908404