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Fertilizing ability of gametes at different post‐activation times and the sperm–oocyte ratio in the artificial reproduction of pikeperch Sander lucioperca

Kristan, Jiri, Zarski, Daniel, Blecha, Miroslav, Policar, Tomas, Malinovskyi, Oleksandr, Samarin, Azin Mohagheghi, Palinska‐Zarska, Katarzyna, Nowosad, Joanna, Krejszeff, Slawomir, Kucharczyk, Dariusz
Aquaculture research 2018 v.49 no.4 pp. 1383-1388
Sander lucioperca, hatcheries, in vitro fertilization, males, milt, oocytes, spermatozoa
The time period during which oocyte and spermatozoa retain their fertilizing ability after contacting with water was evaluated in pikeperch (Sander lucioperca). In addition, success of in vitro fertilization was examined regarding to the sperm‐to‐oocyte ratio (SOR). In the first trial, oocytes were placed in Petri dishes containing 5 ml of the hatchery water, to which freshly collected and pooled sperm were added to each sample at 0, 15, 30, 60, 90, 120, 150 and 180 s post oocyte activation. The oocytes retained their fertility for at least 30 s after contacting with water. The second trial tested the maximum time period during which spermatozoa retained fertilizability after contacting with water. Milt (50 μl) was collected from each male and added to 5 ml of water in Petri dishes. Thereafter, oocytes were added at 0, 5, 15, 30, 60 and 75 s post‐sperm activation. Delays exceeding 10 s affected negatively the fertilization success. The third trial examined the optimum SOR; in which was found that 100 × 10³ spermatozoa per oocyte were the minimum ratio to ensure fertilization rates above 70%. Overall, the data clarified some biological interactions of gametes in the artificial propagation of pikeperch.