Jump to Main Content
Optimization of Cellulase Production by Trichoderma Strains Using Crude Glycerol as a Primary Carbon Source with a 24 Full Factorial Design
- de Sousa, KallyAlves, da Faheina Junior, GeniltonSilva, de Azevedo, DianaCristina Silva, Pinto, GustavoAdolfo Saavedra
- Waste and biomass valorization 2018 v.9 no.3 pp. 357-367
- Trichoderma, ammonium sulfate, carbon, cellulose, correlation, culture media, endo-1,4-beta-glucanase, fermentation, glycerol, regression analysis, yeast extract
- This work focuses on the optimization of cellulase production by two Trichoderma strains. A 2⁴ full factorial design was used to evaluate the effects of four factors in the optimization of cellulase production (filter paper assay—FPA): crude glycerol, microcrystalline cellulose, yeast extract and ammonium sulfate. In fermentation with Trichoderma CMIAT 054 strain the largest FPA (138.48 FPU L⁻¹) occurred with 25.0 g L⁻¹ of cellulose, 10.0 g L⁻¹ of crude glycerol, 1.4 g L⁻ ¹ of yeast extract and 3.5 g L⁻ ¹ of ammonium sulfate in the culture medium. In tests with Trichoderma CMIAT 041 strain the highest FPA (89.35 FPU L⁻¹) occurred with 25.0 g L⁻¹ of cellulose, 20.0 g L⁻¹ of crude glycerol, 0.6 g L⁻¹ of yeast extract and 1.5 g L⁻¹ of ammonium sulfate in the culture medium. ANOVA showed a correlation coefficient of 93 and 88% for Trichoderma CMIAT 054 and CMIAT 041 strains, respectively. Reduced regression models for the cellulase produced by these strains were obtained.