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Identification of quantitative trait loci associated with drought tolerance traits in rice (Oryza sativa L.) under PEG and field drought stress

Author:
Han, Bing, Wang, Jiao, Li, Yafei, Ma, Xiaoding, Jo, Sumin, Cui, Di, Wang, Yanjie, Park, Dongsoo, Song, Youchun, Cao, Guilan, Wang, Xingsheng, Sun, Jianchang, Shin, Dongjin, Han, Longzhi
Source:
Euphytica 2018 v.214 no.4 pp. 74
ISSN:
0014-2336
Subject:
Oryza sativa, chromosomes, drought tolerance, environmental impact, germination, inbred lines, leaves, marker-assisted selection, phenotype, phenotypic variation, plant breeding, polyethylene, quantitative trait loci, rice, seedlings, vigor, water stress
Abstract:
Two recombinant inbred line F₁₀ rice populations (IAPAR-9/Akihikari and IAPAR-9/Liaoyan241) were used to identify quantitative trait loci (QTLs) for ten drought tolerance traits at the budding and early seedling stage under polyethylene glycol-induced drought stress, and two traits of leaf rolling index (LRI) and leaf withering degree (LWD) under field drought stress. The results showed that the drought-tolerance capacity of IAPAR-9 was stronger than that of Akihikari and Liaoyan241. Thirty-four QTLs for 12 drought tolerance traits were detected, and among them, in the IAPAR-9/Akihikari population, qLRI9-1 and qLRI10-1 for LRI were repeatedly detected in RM3600-RM553 on chromosome 9 and in RM6100-RM3773 on chromosome 10, respectively, at two times points of July 31 and August 13 in 2014. The two QTLs are stable against the environmental impact, and qLRI9-1 and qLRI10-1 explained 6.77–13.66% and 5.01–8.32% of the phenotypic variance, respectively, at the two times points. qLWD9-2 for LWD in the IAPAR-9/Liaoyan241 population contributed 8.73% of variation was detected in the same marker interval with the qLRI9-1, and qLRI1-1 for LRI and qLWD1-1 for LWD were located in the same marker interval RM11054-RM5646 on chromosome 1, which contributed 18.82 and 5.78% of phenotype variation respectively. qGV3 for germination vigor and qRGV3 for relative germination vigor at the budding stage were detected in the same marker interval RM426-RM570 on chromosome 3, which explained 14.98 and 16.30% of the observed phenotypic variation respectively, representing major QTLs. The above-mentioned stable or major QTLs regions could be useful for molecular marker assisted selection breeding, fine mapping, and cloning.
Agid:
5913384