Jump to Main Content
Chemical composition, in vitro anti-tumor activities and related mechanisms of the essential oil from the roots of Potentilla discolor
- Zhang, Jing, Huang, Ri-Zhen, Cao, Hong-Jie, Cheng, An-Wei, Jiang, Cheng-Shi, Liao, Zhi-Xin, Liu, Chao, Sun, Jin-Yue
- Industrial crops and products 2018 v.113 pp. 19-27
- DNA replication, Oriental traditional medicine, Potentilla, antineoplastic activity, antineoplastic agents, apoptosis, carbon dioxide, cell cycle, cell growth, cell lines, essential oils, gas chromatography-mass spectrometry, ingredients, inhibitory concentration 50, neoplasm cells, neoplasms, pharmaceutical industry, roots, supercritical fluid extraction
- Potentilla discolor has been used as the Traditional Chinese Medicine ingredient in prescription for over 400 years, and scientific evidences have confirmed its effectiveness as an anticancer agent. The present study is undertaken to assess, for the first time, the chemical composition, potential in vitro anti-tumor activities and related mechanisms of the essential oil, which was extracted from Potentilla discolor fresh roots by supercritical CO2 fluid extraction technology. Twenty-nine compositions were identified by gas chromatography-mass spectrometry. The anti-tumor activity of the essential oil was screened against four cancer cell lines and the lowest IC50 value was 19.02 μg/mL on T24 cell line. Then, T24 was selected as a representative cell line for the related mechanism research. As a result, cell cycle assay confirmed that the cell growth was inhibited by Potentilla discolor essential oil and cell cycle was arrested in DNA synthesis phase. A series of apoptosis analysis indicated that Potentilla discolor essential oil induced apoptosis through mitochondrion-mediated intrinsic pathway. This study revealed that Potentilla discolor essential oil has significant anti-tumor activity. It should be useful in the search for new potential anti-tumor agents and pharmaceutical industries.