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Pandoraea sp. B-6 assists the deep eutectic solvent pretreatment of rice straw via promoting lignin depolymerization

Author:
Liu, Dan, Yan, Xu, Zhuo, Shengnan, Si, Mengying, Liu, Mingren, Wang, Sheng, Ren, Lili, Chai, Liyuan, Shi, Yan
Source:
Bioresource technology 2018 v.257 pp. 62-68
ISSN:
0960-8524
Subject:
Pandoraea, biorefining, depolymerization, droplets, enzymatic hydrolysis, lignin, lignocellulose, reducing sugars, rice straw, solvents, yields
Abstract:
Lignin depolymerization is a challenging process in biorefinery due to the recalcitrant and complex structure of lignin. This challenge was herein addressed via elaborating a new strategy of combining the bacterial strain Pandoraea sp. B-6 (hereafter B-6) with a deep eutectic solvent (DES) to pretreat rice straw (RS). In this approach, DES effectively depolymerized lignin yet easily caused sugar loss under severe conditions. B-6 not only overcame the obstacle of lignin droplets, but also significantly improved enzymatic digestibility. After B-6 assisted DES pretreatment, the reducing sugar yield increases by 0.3–1.5 times over DES pretreatment and 0.9–3.1 times over the untreated RS. Furthermore, a “cornhusking” mechanism explaining the improvement of the enzymatic digestibility by B-6 was suggested based on physicochemical characterizations of the untreated and pretreated RS. The findings provided a comprehensive perspective to establish a DES−microbial process for lignocellulose pretreatment.
Agid:
5914515