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The antioxidant and neurochemical activity of Apium graveolens L. and its ameliorative effect on MPTP-induced Parkinson-like symptoms in mice

Author:
Chonpathompikunlert, Pennapa, Boonruamkaew, Phetcharat, Sukketsiri, Wanida, Hutamekalin, Pilaiwanwadee, Sroyraya, Morakot
Source:
BMC complementary and alternative medicine 2018 v.18 no.1 pp. 103
ISSN:
1472-6882
Subject:
Apium graveolens, Oriental traditional medicine, Parkinson disease, alternative medicine, amine oxidase (flavin-containing), antioxidant activity, diabetes, drugs, gout, hypertension, immunohistochemistry, lipid peroxidation, males, mice, models, neurons, neuroprotective effect, neurotransmitters, oxidative stress, staining, superoxide anion, swimming, tyrosine 3-monooxygenase
Abstract:
BACKGROUND: Apium graveolens L. is a traditional Chinese medicine prescribed as a treatment for hypertension, gout, and diabetes. This study aimed to determine the neuroprotective effects of A. graveolens extract against a Parkinson’s disease (PD) model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL/6 mice. METHODS: Male C57BL/6 mice treated with MPTP were orally dosed with A. graveolens extract daily for 21 days. Behavioral tests, including a rotarod apparatus, a narrow beam test, a drag test, a grid walk test, a swimming test, and a resting tremor evaluation, were performed. Thereafter, the mice were sacrificed, and monoamine oxidase A and B activity, lipid peroxidation activity, and superoxide anion levels were measured. Immunohistochemical staining of tyrosine hydroxylase was performed to identify dopaminergic neurons. RESULTS: We found that treatment with A. graveolens at dose of 375 mg/kg demonstrated the highest effect and led to significant improvements in behavioral performance, oxidative stress parameters, and monoamine oxidase A and B activity compared with the untreated group (p < 0.05). Moreover, the extract increased the number of neurons immunopositive for tyrosine hydroxylase expression compared with MPTP alone or MPTP with a positive control drug (p < 0.05). CONCLUSIONS: We speculated that A. graveolens ameliorated behavioral performance by mediating neuroprotection against MPTP-induced PD via antioxidant effects, related neurotransmitter pathways and an increase in the number of dopaminergic neurons.
Agid:
5916658