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Characterization, anti-oxidation and anti-inflammation of polysaccharides by Hypsizygus marmoreus against LPS-induced toxicity on lung

Liu, Min, Li, Shangshang, Wang, Xiuxiu, Zhu, Yongfa, Zhang, Jianjun, Liu, Hui, Jia, Le
International journal of biological macromolecules 2018 v.111 pp. 121-128
C-reactive protein, Hypsizygus marmoreus, antioxidant activity, antioxidants, blood serum, catalase, complement, enzyme activity, gamma-glutamyltransferase, glutathione peroxidase, histopathology, interleukin-1beta, lipid peroxidation, lipopolysaccharides, lungs, malondialdehyde, mice, mycelium, myeloperoxidase, protective effect, superoxide dismutase, toxicity, tumor necrosis factor-alpha
In our present work, the polysaccharides (MPS) were successfully extracted from the mycelia of Hypsizygus marmoreus, and purpose of our work was to evaluate the analysis of the characterization, anti-inflammation and anti-oxidation against the lung failure induced by lipopolysaccharide (LPS) in mice. Pretreatment with MPS at 800mg/kg markedly ameliorated the lung wet-to-dry weight (W/D) ratio and pulmonary histopathological conditions, reduced the tumor necrosis factor-alpha (TNF)-α, interleukin (IL)-6, and IL-1β levels in bronchoalveolar lavage fluid (BALF), myeloperoxidase (MPO) activities. In addition, the protective effects of MPS might be attributed to the down-regulations of the serum complement 3 (C3), glutamyl transpeptidase (GGT) and high-sensitivity C-reactive protein (hs-CPR) contents, as well as improving the antioxidant status by enhancing pulmonary enzyme activities (catalase (CAT), superoxide dismutase(SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC)) and eliminating the lipid peroxidation (LPO) and malondialdehyde (MDA), respectively. What's more, our work investigated the characterization of the MPS.