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Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Author:
Belhaj, Dalel, Athmouni, Khaled, Ahmed, Mohammad Boshir, Aoiadni, Nissaf, El Feki, Abdelfattah, Zhou, John L., Ayadi, Habib
Source:
International journal of biological macromolecules 2018 v.113 pp. 813-820
ISSN:
0141-8130
Subject:
DNA damage, Phormidium, antioxidant activity, antioxidants, cadmium, cytotoxicity, enzyme activity, hepatoma, hepatoprotective effect, hepatotoxicity, humans, in vitro studies, in vivo studies, liver, molecular weight, neoplasm cells, polysaccharides, rats, tissues
Abstract:
The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25–150μg·mL−1) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P<0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.
Agid:
5917212