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High-value application of cassava starch in root crop micropropagation for sustainable seed systems in developing countries
- Acedo, V. Z., Hinay, N. G., Binongo, M. M., Antona, J. Q.
- Acta horticulturae 2017 no.1179 pp. 293-298
- Ipomoea batatas, Manihot esculenta, agar, cassava, cassava starch, crop production, cultivars, culture media, developing countries, growth and development, micropropagation, planting, root growth, roots, shoots, starch, yams
- Despite the high cost of micropropagation, the use of tissue-cultured planting materials is now widely practiced in several crop production ventures in developing countries. One of the high-cost items in micropropagation is the agar as gelling agent of the culture medium, which is imported. This study examined the feasibility of using locally available cassava starch as a low-cost agar substitute in the micropropagation of purple yam and cassava. Starches from two cassava cultivars ('Lakan-1' and 'Rayong-5') were tested at different levels. Results showed normal growth and development of purple yam nodal cultures at all levels of starch used. However, at 8% starch, the medium was so soft that the nodal tissues were submerged, thereby causing a delay in shoot and root growth. Starch concentrations of 9% in both cultivars appeared to be optimal for yam, wherein culture responses were comparable to that of the usual 0.7% agar. However, for cassava, regardless of concentration, both starches were found to be unsuitable, because the cultures did not exhibit normal growth and development. Cost analysis of the use of 9% cassava starch revealed a more than 10-fold saving relative to the use of 0.7% agar.