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A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology
- Miller, Christopher N., Jossé, Lyne, Brown, Ian, Blakeman, Ben, Povey, Jane, Yiangou, Lyto, Price, Mark, Cinatl, Jindrich, Xue, Wei-Feng, Michaelis, Martin, Tsaousis, Anastasios D.
- International journal for parasitology 2018 v.48 no.3-4 pp. 197-201
- Cryptosporidium parvum, cell culture, children, diarrhea, immunocompromised population, models, oocysts, parasites, vaccines
- Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children in developing areas and immunocompromised individuals. Curative therapies and vaccines are lacking, mainly due to lack of a long-term culturing system of this parasite. Here, we show that COLO-680N cells infected with two different Cryptosporidium parvum strains produce sufficient infectious oocysts to infect subsequent cultures, showing a substantial fold increase in production, depending on the experiment, over the most optimistic HCT-8 models. Oocyst identity was confirmed using a variety of microscopic- and molecular-based methods. This culturing system will accelerate research on Cryptosporidium and the development of anti-Cryptosporidium drugs.