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Evaluation of carbon nanotube based copper nanoparticle composite for the efficient detection of agroviruses
- Muhammad Ali Tahir, Sadia Z. Bajwa, Shahid Mansoor, Rob W. Briddon, Waheed S. Khan, Brian E. Scheffler, Imran Amin
- Journal of hazardous materials 2018 v.346 pp. 27-35
- DNA, Geminiviridae, Gossypium, biosensors, carbon nanotubes, copper nanoparticles, cotton, detection limit, electrochemistry, hybridization, leaves, methylene blue, monitoring, nanocomposites, sap, viruses, Africa, Asia
- We report a biosensor that combines the construction of a three-dimensional nanocomposite with electrochemical methods for the detection of viruses in plants. This is the first report, where carbon nanotubes are used as a conductive frame to anchor highly electrolytic agglomerates of copper nanoparticles to detect agroviruses. Morphological analysis of nanocomposite revealed the presence of carbon nanotubes having a diameter of 50–100nm with copper nanoparticles of 20–100nm, attached in the form of bunches. This material was applied to assess the infection caused by geminiviruses which are a major threat to the cotton plants in Asian and African countries. The hybridization events were studied by monitoring differential pulse voltammetry signals using methylene blue as a redox indicator. In the presence of target DNA, sensor signals decreased from 7×10⁻⁴ to 1×10⁻⁴Ampere. The probe exhibited 97.14% selectivity and the detection limit was found to be 0.01ngμL⁻¹. The developed biosensor is stable for at least four weeks, losing only 4.3% of the initial signal value. This sensor was able to detect the presence of viruses in sap extracted from cotton leaves, thus providing a promising platform to detect a range of other crops-infecting viruses.