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HPLC analysis of six iridoid glycosides from Cymbaria dahurica L. and their structural elucidation

Wang, Qinghu, Bao, Yinping, Hao, Junsheng, Han, Jingjing
Journal of food biochemistry 2018 v.42 no.2 pp. e12470
absorbance, acetonitrile, aerial parts, antioxidant activity, antioxidants, ethanol, flavonoids, foods, high performance liquid chromatography, iridoid glycosides, medicinal properties, secondary metabolites, statistical analysis, China
Studies on ethanol extract from Cymbaria dahurica L. (C. dahurica) resulted in isolation and identification of a new iridoid glycoside together with five known compounds. Moreover, a high‐performance liquid chromatographic method has been developed for the simultaneous determination of six iridoid glycosides in C. dahurica. The separation by gradient elution was performed on Inertsil ODS‐SP column (250 mm × 4.6 mm, 5 µm) at 30°C with acetonitrile and water as the mobile phase, and monitored by absorbance at 210 nm. The recovery of the method is from 95.0 to 98.0%, and linearity (r > .9994) was obtained for six compounds. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.6%) were also achieved. The validated method was successfully applied to the determination of six compounds in six samples. PRACTICAL APPLICATIONS: The aerial parts of Cymbaria dahurica has been used as foods and herbal remedies in Inner Mongolia of China, which has a wide range of health benefits, significantly antioxidant properties. In previous phytochemical studies, non‐glycosidic iridoids and flavonoids were isolated. Iridoids are a class of secondary metabolites with the significant pharmacological activities including antitumor, antioxidant, and anti‐inflammatory. Therefore, a detailed analysis and recognition of structural compounds diversity in C. dahurica is of great importance to evaluate their biological activities.