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A non-toxic microbial surfactant from Marinobacter hydrocarbonoclasticus SdK644 for crude oil solubilization enhancement

Author:
Zenati, Billal, Chebbi, Alif, Badis, Abdelmalek, Eddouaouda, Kamel, Boutoumi, Hocine, El Hattab, Mohamed, Hentati, Dorra, Chelbi, Manel, Sayadi, Sami, Chamkha, Mohamed, Franzetti, Andrea
Source:
Ecotoxicology and environmental safety 2018 v.154 pp. 100-107
ISSN:
0147-6513
Subject:
Artemia, Fourier transform infrared spectroscopy, Marinobacter hydrocarbonoclasticus, acute toxicity, bioactive compounds, bioassays, bioremediation, biosurfactants, carbon, frying oil, gas chromatography-mass spectrometry, glycolipids, larvae, marine environment, nucleotide sequences, petroleum, phylogeny, polysorbates, ribosomal RNA, seawater, sequence homology, solubilization, wastes
Abstract:
This study aims to investigate the ability of a biosurfactant produced by Marinobacter hydrocarbonoclasticus strain SdK644 isolated from hydrocarbon contaminated sediment to enhance the solubilization rate of crude oil contaminated seawater. Phylogenetic analysis shows that strain SdK644 was very closely related to M. hydrocarbonoclasticus with 16S rRNA gene sequence similarity of 97.44%. Using waste frying oil as inducer carbon source, the producing biosurfactant by strain SdK644 was applied to improve crude oil solubilization in seawater. The preliminary characterization of the produced biosurfactant by FT-IR analysis indicates its possible classification in a glycolipids group. Results from crude oil solubilization assay showed that SdK644 strain biosurfactant was 2-fold greater than Tween 80 surfactant in crude oil solubilization and 12-fold higher than seawater control, as shown by GC-MS analysis of aliphatic compounds. Furthermore, this bioactive compound was shown to be nontoxic against Artemia larvae in short-term acute toxicity bioassay. Generally, the results showed the possible use of M. hydrocarbonoclasticus strain SdK644 biosurfactant in bioremediation processes of the marine environments.
Agid:
5923057