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Urinary bladder organ hypertrophy is partially regulated by Akt1-mediated protein synthesis pathway

Qiao, Li-Ya, Xia, Chunmei, Shen, Shanwei, Lee, Seong Ho, Ratz, Paul H., Fraser, Matthew O., Miner, Amy, Speich, John E., Lysiak, Jeffrey J., Steers, William D.
Life sciences 2018 v.201 pp. 63-71
Western blotting, actin, bladder, collagen, fibrosis, hypertrophy, messenger RNA, muscles, phosphatidylinositol 3-kinase, phosphorylation, proliferating cell nuclear antigen, protein synthesis, quantitative polymerase chain reaction, rapamycin, rats, smooth muscle
The present study aims to investigate the role of Akt in the regulation of urinary bladder organ hypertrophy caused by partial bladder outlet obstruction (pBOO).Male rats were surgically induced for pBOO. Real-time PCR and western blot were used to examine the levels of mRNA and protein. A phosphoinositide 3-kinase (PI3K) inhibitor LY294002 was used to inhibit the activity of endogenous Akt.The urinary bladder developed hypertrophy at 2 weeks of pBOO. The protein but not mRNA levels of type I collagen and α-smooth muscle actin (αSMA) were increased in pBOO bladder when compared to sham control. The phosphorylation (activation) levels of Akt1 (p-Ser⁴⁷³), mammalian target of rapamycin (mTOR), p70S6 kinase (p70S6K), and 4E-BP1 were also increased in pBOO bladder. LY294002 treatment reduced the phosphorylation levels of Akt1 and 4E-BP1, and the protein levels of type I collagen and αSMA in pBOO bladder. The mRNA and protein levels of proliferating cell nuclear antigen (PCNA) were increased in pBOO bladder, and PCNA up-regulation occurred in urothelial not muscular layer. LY294002 treatment had no effect on the mRNA and protein levels of PCNA in pBOO bladder. LY294002 treatment partially reduced the bladder weight caused by pBOO.pBOO-induced urinary bladder hypertrophy is attributable to fibrosis, smooth muscle cellular hypertrophy, and urothelium cell hyper-proliferation. Akt1-mediated protein synthesis in pBOO bladder contributes to type I collagen and αSMA but not PCNA up-regulation. Target of Akt1 is necessary but not sufficient in treatment of urinary bladder hypertrophy following pBOO.