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Development of a new analytical tool for assessing the mutagen 2-methyl-1,4-dinitro-pyrrole in meat products by LC-ESI-MS/MS

Author:
Molognoni, Luciano, Daguer, Heitor, de Sá Ploêncio, Leandro Antunes, Yotsuyanagi, Suzana Eri, da Silva Correa Lemos, Ana Lucia, Joussef, Antonio Carlos, De Dea Lindner, Juliano
Source:
Talanta 2018 v.185 pp. 151-159
ISSN:
0039-9140
Subject:
bacon, hydroxylation, liquid chromatography, meat processing, mutagens, nitrites, sausages, silane, silica, tandem mass spectrometry, temperature
Abstract:
The use of sorbate and nitrite in meat processing may lead to the formation of 2-methyl-1,4-dinitro-pyrrole (DNMP), a mutagenic compound. This work was aimed at developing and validating an analytical method for the quantitation of DNMP by liquid chromatography-tandem mass spectrometry. Full validation was performed in accordance to Commission Decision 2002/657/EC and method applicability was checked in several samples of meat products. A simple procedure, with low temperature partitioning solid-liquid extraction, was developed. The nitrosation during the extraction was monitored by the N-nitroso-DL-pipecolic acid content. Chromatographic separation was achieved in 8 min with di-isopropyl-3-aminopropyl silane bound to hydroxylated silica as stationary phase. Samples of bacon and cooked sausage yielded the highest concentrations of DNMP (68 ± 3 and 50 ± 3 μg kg−1, respectively). The developed method proved to be a reliable, selective, and sensitive tool for DNMP measurements in meat products.
Agid:
5925778