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Production of 6-O-L-Ascorbyl Palmitate by Immobilized Candida antarctica Lipase B
- Yadav, ManishG., Kavadia, MonaliR., Vadgama, RajeshkumarN., Odaneth, AnnammaA., Lali, ArvindM.
- Applied biochemistry and biotechnology 2018 v.184 no.4 pp. 1168-1186
- Fourier transform infrared spectroscopy, Pseudozyma antarctica, alcohols, ascorbic acid, batch systems, byproducts, carboxylic ester hydrolases, catalysts, cosmetics, energy, hygiene, industry, palmitates, palmitic acid, wastes
- L-ascorbyl palmitate (ASP) is an oil-soluble derivative of ascorbic acid which is used extensively in food, cosmetics industry, and medical hygiene. Enzymatic synthesis of ascorbyl palmitate in tert-butyl alcohol was carried out using indigenously immobilized lipase preparation PyCal with ascorbic acid and palmitic acid as starting material. The developed batch process under optimized reaction conditions resulted in conversion of 90% with relatively shorter reaction time of 6 h. Continuous process in packed bed reactor gave conversion of 50% with space time yield of 15.46 g/L/h which was found to be higher than the reported literature on enzymatic synthesis of ascorbyl palmitate. The immobilized lipase used in the present work showed good reusability. Characterization of formed ascorbyl palmitate was carried out by FTIR, MS/MS, H¹-NMR, and C¹³-NMR. The enzymatic process resulted in selective synthesis of 6-O-L-ascorbyl palmitate with purity of 98.6% and no side product formation. The use of underivatized starting materials, high space time yield of 15.46 g L⁻¹ h⁻¹, high recyclability of catalyst, and no by-product formation make the overall process highly efficient and clean in terms of energy consumption and waste generation, respectively. The optimized reaction parameters for ascorbyl palmitate synthesis in the present study can be used as a useful reference for industrial synthesis of fatty acid esters of ascorbic acid by enzymatic route.