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Salibacter halophilus gen. nov., sp. nov., isolated from a saltern

Lu, De-Chen, Xia, Jun, Dunlap, Christopher A., Rooney, Alejandro P., Du, Zong-Jun
International journal of systematic and evolutionary microbiology 2017 v.67 pp. 1784-1788
Flavobacteriales, Gram-negative bacteria, anaerobes, fatty acids, genes, halophiles, new genus, new species, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, quinones, ribosomal DNA, ribosomal RNA, sequence homology, sodium chloride, China
A Gram-stain-negative and facultatively anaerobic bacterium, JZ3C34T, was isolated from a saltern near Feicheng, China (36°8'24.45" E, 116°49'22.46" N). Cells of strain JZ3C34T were 0.3-0.4µm wide and 1.5-2.0µm long, catalase-positive and oxidase-negative. Colonies were transparent, orange and approximately 1.0 mm in diameter. Growth occurred at 20-50 °C (optimally at 33-37 °C), pH 6.5-8.5 (optimally at 7.0-8.0) and in the presence of 2-18 % (w/v) NaCl (optimally in 6-8 % (w/v) NaCl). Phylogenetic analysis of the 16S rRNA gene indicated that strain JZ3C34T was a member of the family Cryomorphaceae within the order Flavobacteriales and the most closely related species was Owenweeksia hongkongensis DSM 17368T, although the relationship was fairly distant and consistent with the taxa being members of different genera (89.2 % 16S rRNA gene sequence similarity). The major respiratory quinone of strain JZ3C34T was found to be MK-7, and the dominant fatty acids were found to be iso-C15:0 and iso-C15:1 G. The major polar lipids were two kind of unidentified lipids (L2 and L3) and phosphatidylethanolamine (PE), and the genomic DNA G+C content was 39.6 mol %. The polyphasic taxonomy traits presented here clearly place the new strain as a novel species within a new genus of the family Cryomorphaceae, for which we propose the name Salimarina halophila gen. nov., sp. nov. The type strain is JZ3C34T (=KCTC 52047T=MCCC 1K02288T).