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Biodegradation of bensulfuron-methyl and its effect on bacterial community in paddy soils

Lin, Xiao-Yan, Yang, Yu-Yi, Zhao, Yu-Hua, Fu, Qing-Lin
Ecotoxicology 2012 v.21 no.5 pp. 1281-1290
Bacillus megaterium, bacterial communities, bensulfuron-methyl, biodegradation, broadleaf weeds, community structure, denaturing gradient gel electrophoresis, paddies, paddy soils, soil bacteria
Bensulfuron-methyl (BSM) is a new kind of sulfonylurea herbicide widely used to control broad-leaf weeds in rice paddies. The aim of this work was to study BSM biodegradation in paddy soils with BSM-degrading bacteria Bacillus megaterium L1 and Brevibacterium sp. BH and its effect on the structures of soil bacterial community. More than 90 % of BSM could be degraded in paddy soils with 0.0355 mg kg−1 BSM concentration. Addition of BSM-degrading bacterial strains Bacillus megaterium L1 into BSM contaminated paddy soil could have the half-life time of BSM compared to treatment without Bacillus megaterium L1 inoculation. Denaturing gradient gel electrophoresis and principle component analysis indicated that the diversity of the soil microbial community structure changed along with the addition of BSM, which recovered at the end of the experiment (5 weeks). Addition of BSM-degrading bacteria Bacillus megaterium L1 enriched the diversity of soil microbial community structure in paddy soils. This study provides information on the biodegradation of BSM and BSM’s influences on the soil bacteria microbial community structures.