U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

A recombinant flagellin fragment, which includes the epitopes flg22 and flgII-28, provides a useful tool to study flagellin-triggered immunity

Author:
Serena Ciarroni, Christopher R. Clarke, Haijie Liu, Noam Eckshtain-Levi, Angelo Mazzaglia, Giorgio M. Balestra, Boris A. Vinatzer
Source:
Journal of general plant pathology 2018 v.84 no.3 pp. 169-175
ISSN:
1345-2630
Subject:
Actinidia arguta, Arabidopsis thaliana, Pseudomonas syringae, amino acids, animals, epitopes, flagellin, flagellum, gene overexpression, innate immunity, kiwifruit, leaves, oxygen, plants (botany)
Abstract:
Plants and animals independently evolved the ability to recognize flagellin (also called FliC), the building block of the bacterial flagellum, as part of their innate immune response. While animals recognize a relatively large region of FliC, most plants recognize one or two short epitopes of FliC: flg22 and flgII-28. However, since most research in plants has focused on flg22 and flgII-28 and not the actual FliC protein, the importance of any FliC region beyond the two epitopes in plant immunity is poorly understood. Here we report cloning, overexpression, and purification of a Pseudomonas syringae FliC fragment from amino acid 1 to 143, which includes both FliC epitopes and the adjacent alpha helices. Exposing Arabidopsis thaliana leaves to FliC₁–₁₄₃ did not reveal any additional FliC recognition capabilities beyond flg22. However, while the kiwifruit species Actinidia arguta did not respond to either flg22 or flgII-28, treatment of A. arguta leaves with FliC₁–₁₄₃ triggered a significant reactive oxygen response, indicating recognition. This result suggests that in some plant species, recognition of FliC requires regions of FliC beyond the two well-known epitopes and that FliC₁–₁₄₃ represents a useful tool in the study of plant immunity.
Agid:
5928776
Handle:
10113/5928776