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Annotation and De Novo Sequence Characterization of Extracellular β-Fructofuranosidase from Penicillium chrysogenum Strain HKF42

Author:
Gujar, VaibhavV., Fuke, Priya, Khardenavis, AnshumanA., Purohit, HemantJ.
Source:
Indian journal of microbiology 2018 v.58 no.2 pp. 227-233
ISSN:
0046-8991
Subject:
Penicillium chrysogenum, beta-fructofuranosidase, enzyme activity, fungi, genes, kestose, nystose, prebiotics, sucrose
Abstract:
The genome of a fungal strain Penicillium chrysogenum strain HKF42, which can grow on 20% sucrose has been annotated for 7595 protein coding sequences. On mining of CAZymes, we could annotate a β-fructofuranosidase gene responsible for fructo-oligosaccharides (FOS) synthesis which is a known prebiotic. The enzyme activity was demonstrated and validated with the generation of FOS as kestose and nystose.
Agid:
5929132