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The polystyrene-divinylbenzene stationary phase hybridized with oxidized nanodiamonds for liquid chromatography

Huang, Zhongping, Yao, Peng, Zhu, Qiulian, Wang, Lili, Zhu, Yan
Talanta 2018 v.185 pp. 221-228
Fourier transform infrared spectroscopy, X-radiation, X-ray diffraction, X-ray photoelectron spectroscopy, anions, benzaldehyde, benzoic acid, bromides, chlorides, fluorides, methylamine, microparticles, nanodiamonds, nitrates, oxidation, pH, phenol, phosphates, polymerization, polymers, quinones, reversed-phase liquid chromatography, scanning electron microscopy, sulfates, thermogravimetry, toluene, transmission electron microscopes, transmission electron microscopy
A novel polystyrene-divinylbenzene microspheres hybridized with oxidized nanodiamonds (PS-DVB-OND) was synthesized by the method of seed swelling and polymerization. The oxidized nanodiamonds (OND) were characterized by Fourier transform infrared (FTIR) spectra, X-ray phtoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), X-ray diffraction (XRD). PS-DVB-OND particles were characterized by scanning electron microscopy (SEM) and transmission electron microscope (TEM). The result suggested that OND were successfully embedded into the polymer microspheres with the diameter of 6 ± 2 µm. Compared to polystyrene-divinylbenzene (PS-DVB) microspheres, PS-DVB-OND microspheres could tolerate higher pressure. The PS-DVB-OND microspheres were used as stationary phase of reversed-phase liquid chromatography directly and anion-exchangers after further quaternized with methylamine and 1,4-butanediol diglycidyl ether. Reversed-phase liquid chromatographic performance of PS-DVB-OND beads was investigated through separating six benzenes such as toluene, benzaldehyde, phenol, benzoic acid, 1,4-hydroquinone and methyl p-hydroxybenzoate. Inorganic anions such as F-, Cl-, NO2-, Br-, NO3-, HPO42- and SO42-, were baseline separated on the anion exchangers of PS-DVB-OND microspheres. The result suggested that the prepared PS-DVB-OND microspheres have the potential as liquid chromatographic stationary phase under high pressure and extremely pH conditions.