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Effects of cadmium on calcium homeostasis in the white-rot fungus Phanerochaete chrysosporium

Zhang, Xiaoxiao, Shao, Jihai, Chen, Anwei, Shang, Cui, Hu, Xinjiang, Luo, Si, Lei, Ming, Peng, Liang, Zeng, Qingru
Ecotoxicology and environmental safety 2018 v.157 pp. 95-101
Phanerochaete chrysosporium, bioremediation, cadmium, calcium, calcium channels, cell viability, homeostasis, hormesis, pollutants, white-rot fungi
Due to the widespread application of white-rot fungi for the treatment of pollutants, it's crucial to exploit the special effects of pollutants on the microbes. Here, we studied the effects of cadmium on calcium homeostasis in the most studied white-rot fungus Phanerochaete chrysosporium. The response of P. chrysosporium to cadmium stress is concentration-dependent. A high concentration of cadmium caused the release of calcium from P. chrysosporium, while a hormesis effect was observed at a lower cadmium concentration (10 μM), which resulted in a significant increase in calcium uptake and reversed the decrease in cell viability. Calcium (50 μM) promoted cell viability (127.2% of control), which reflects that calcium can protect P. chrysosporium from environmental stress. Real-time changes in the Ca²⁺ and Cd²⁺ fluxes of P. chrysosporium were quantified using the noninvasive microtest technique. Ca²⁺ influx decreased significantly under cadmium exposure, and the Ca²⁺ channel was involved in Ca²⁺ and Cd²⁺ influx. The cadmium and/or calcium uptake results coupled with the real-time Ca²⁺ and Cd²⁺ influxes microscale signatures can enhance our knowledge of the homeostasis of P. chrysosporium with respect to cadmium stress, which may provide useful information for improving the bioremediation process.