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Longibacter salinarum gen. nov., sp. nov., isolated from a marine solar saltern

Jun Xia, Christopher A. Dunlap, Lina Flor-Weiler, Alejandro P. Rooney, Guan-Jun Chen, Zong-Jun Du
International journal of systematic and evolutionary microbiology 2016 v.66 no.9 pp. 3287-3292
Gram-negative bacteria, Rhodothermaceae, anaerobes, arabinose, cellobiose, coasts, fatty acids, fructose, galactose, glucose, glycerol, glycolipids, halophiles, high performance liquid chromatography, maltose, mannitol, mannose, naphthoquinones, new genus, new species, nitrates, nucleotide sequences, pH, phenotype, phosphatidylethanolamines, phylogeny, rhamnose, ribosomal DNA, ribosomal RNA, sequence homology, sodium chloride, starch, sucrose, xylose, China
A bacterial strain, designated WDS2C18T, was isolated from a marine solar saltern from the coast of Weihai, Shandong Province, China. Cells of strain WDS2C18T were long rod-shaped, red, and approximately 6.0–12.0 µm in length and 0.3–0.4 µm in width. The strain was Gram-stain-negative, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative. Optimal growth was observed at 40 °C, at pH 7.5–8.0 with 8–12 % (w/v) NaCl. Nitrate was not reduced. Glycerol, sucrose, starch and d-mannitol stimulated growth, but not glucose, d-fructose, d-galactose, d-lactose, maltose, d-mannose, d-xylose, d-ribose, d-arabinose, l-rhamnose or cellobiose. The G+C content of the genomic DNA was 58.1 mol% (HPLC). The sole methyl naphthoquinone was MK-7 and the predominant cellular fatty acids (>10 %) were iso-C15 : 0 2-OH/C16 : 1 ω7c, iso-C17 : 1 ω9c, iso-C15 : 0 and iso-C17 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, glycolipid, three unidentified phospholipids and two unidentified lipids. Phylogenetic analysis, based on 16S rRNA gene sequences, demonstrated that strain WDS2C18T was affiliated with the family Rhodothermaceae . The closest related neighbours were species of the genera Salisaeta andLongimonas; strain WDS2C18T shared highest 16S rRNA gene sequence similarities with Salisaeta longa DSM 21114T (91.7 %) and Longimonas halophila SYD6T (90.8 %) and less than 90.0 % to other species of the family Rhodothermaceae . On the basis of these phenotypic and phylogenetic data, strain WDS2C18T should be classified as representing a novel species of a new genus within the family Rhodothermacea e, for which the name Longibacter salinarum gen. nov., sp. nov. is proposed. The type strain of Longibacter salinarum is WDS2C18T (=MCCC 1H00132T=KCTC 52045T).