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Rhodohalobacter halophilus gen. nov., sp. nov., a moderately halophilic member of the family Balneolaceae

Author:
Xia, Jun, Dunlap, Christopher A., Rooney, Alejandro P., Xie, Zhi-Hong, Du, Zong-Jun
Source:
International journal of systematic and evolutionary microbiology 2017 v.67 no.5 pp. 1281-1287
ISSN:
1466-5034
Subject:
Bacteroidetes, bacteria, fatty acids, glycolipids, lipid composition, menaquinones, new genus, new species, nucleotide sequences, pH, phenotype, phosphatidylethanolamines, phylogeny, quinones, ribosomal DNA, ribosomal RNA, salt tolerance, sodium chloride, China
Abstract:
A novel Gram-stain-negative, rod-shaped, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated JZ3C29T, was isolated from a saltern located in Feicheng, PR China. JZ3C29T was tolerant of moderately saline conditions. Optimal growth occurred at 40 °C (range 20–50 °C) and pH 7.5–8.5 (range pH 7.0–9.0) with 8–10 % (w/v) NaCl (range 2–16 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that JZ3C29T shared highest similarity with Gracilimonas tropica CL-CB462T (90.5 %), Gracilimonas mengyeensis YIM J14T (90.5 %) and Gracilimonas rosea CL-KR2T (90.4 %) and less than 90.0 % similarity with other species of the phylum Bacteroidetes . The isolate formed a novel genus-level clade in the recently described family Balneolaceae . The polar lipid profile of the novel isolate consisted of diphosphatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids, four unidentified phospholipids and two unidentified lipids. The dominant cellular fatty acids (>10 %) were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and iso-C15 : 0 and the sole respiratory quinone was menaquinone 7 (MK-7). The DNA G+C content of JZ3C29T was 44.4 mol%. On the basis of these phenotypic and phylogenetic data, JZ3C29T should be classified as representing a novel genus and species within the family Balneolaceae , for which the name Rhodohalobacter halophilus gen. nov., sp. nov. is proposed. The type strain is JZ3C29T (=MCCC 1H00131T=KCTC 52046T=JCM 31413T).
Agid:
5931562
Handle:
10113/5931562