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Evaluation of a Rapid Test for Antibiotic Residue in Milk Using Spores of Bacillus stearothermophilus var. calidolactis
- Huhtanen, C. N., Phillips, J. G., Brazis, A. R., Bredvold, E., Marshall, R. T., Pederson, D. E., Sing, E. L., Waltz, M. F., Wehr, H. M., Bengsch, H., Thompson, D. I.
- Journal of food protection 1977 v.40 no.4 pp. 236-240
- Geobacillus stearothermophilus, antibiotic residues, beta-lactamase, dried milk, equations, milk, penicillins, prediction, spores
- Eleven analysts tested contaminated reconstituted (1:10) dry milk powders for penicillin residues using spores of Bacillus stearothermophilus var. calidolacris (Delvotest P method). Three types of responses were noted: positive, negative, and questionable. Prediction equations indicated that 95% of the time, analysts unfamiliar with the technique could detect positive results if penicillin concentrations in samples were 0.010 unit/ml or higher and positive and questionable results if the penicillin concentrations were 0.008 unit/ml. Increasing the reconstitution ratio from 1/11 to 1/4 increased the chances of detecting penicillin in milk powder. Penicillinase added to reconstituted penicillin-contaminated milks in all instances produced negative responses.