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Positive effect of resveratrol against preantral follicles degeneration after ovarian tissue vitrification

Rocha, Carina Diniz, Soares, Mayara Mafra, de Cássia Antonino, Deize, Júnior, Jairo Melo, Freitas Mohallem, Renata Ferreira, Ribeiro Rodrigues, Ana Paula, Figueiredo, José Ricardo, Beletti, Marcelo Emílio, Jacomini, José Octavio, Alves, Benner Geraldo, Alves, Kele Amaral
Theriogenology 2018 v.114 pp. 244-251
animal ovaries, cattle, cold tolerance, fetus, follicular development, probability, resveratrol, thawing, viability, vitrification
This study aimed to evaluate whether the addition of resveratrol to vitrification/thawing medium improves the cryotolerance of preantral follicles enclosed in bovine ovarian fragments. Ovarian fragments were obtained from bovine fetuses and distributed to the following groups: fresh ovarian fragments (control), vitrified (VIT), and vitrified with resveratrol (VIT + RESV). Overall, the mean percentage of normal follicles was greater (P < 0.05) in the VIT + RESV compared to the VIT group. Moreover, the probability of finding normal follicles was 2.5 greater (P < 0.05) in the VIT + RESV group. In class comparison, the primordial and transitional follicles have ∼3.0 times (P < 0.05) greater odds of being normal after vitrification compared to the secondary follicles. Additionally, a negative association (P < 0.05) was observed between the proportion of viable follicles and the stage of follicular development. ROS levels were similar (P > 0.05) between the VIT and VIT + RESV groups, and both were lower (P < 0.05) than the control group. The tissue viability in the VIT + RESV group was similar (P > 0.05) to the control group. In summary, the resveratrol provided greater ovarian tissue viability and has a positive effect against degeneration of preantral follicles enclosed in ovarian fragments.