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Radical scavenging activity of sulfated Bupleurum chinense polysaccharides and their effects against oxidative stress-induced senescence
- Tong, Haibin, Zheng, Xiaoli, Song, Jianxi, Liu, Jian, Ren, Ting, Zhang, Xu, Huang, Luqi, Wu, Mingjiang
- Carbohydrate polymers 2018 v.192 pp. 143-149
- 2,2-diphenyl-1-picrylhydrazyl, Bupleurum chinense, Fourier transform infrared spectroscopy, antioxidant activity, beta-galactosidase, cell cycle, cell senescence, drugs, endothelial cells, gas chromatography, high performance liquid chromatography, hydroxyl radicals, immunoblotting, lungs, mice, polysaccharides, staining, sulfates, superoxide anion
- In this study, BCPS-1, a polysaccharide previously isolated and characterized from Bupleurum chinense was chemically modified to yield two sulfated derivatives, which we designated as S-BCP1-4 and S-BCP1-8. The physicochemical properties of these sulfated derivatives were then determined by high performance liquid chromatography (HPLC), Fourier transform infrared spectrometry (FT-IR), and gas chromatography (GC), and then compared with those of BCPS-1. Furthermore, the antioxidant activities of all three polysaccharides were also evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, superoxide radical assay and hydroxyl radical assay, while their effects against H2O2-induced cellular senescence were determined using senescence-associated β-galactosidase (SA-β-gal) staining, cell cycle assay and immunoblotting in H2O2-induced mouse lung endothelial cells (MLECs). Compared to BCPS-1, S-BCP1-4 and S-BCP1-8 exhibited remarkable antioxidant effect, and in a concentration-dependent manner. They also provided stronger protection against H2O2-induced cellular senescence in MLECs. These results indicated that the sulfate group in the modified B. chinense polysaccharides might play an important role in radical scavenging and resistance to H2O2-induced senescence. These sulfated polysaccharides could be considered as novel pharmaceutical products with potential antioxidant and anti-senescence effects.