PubAg

Main content area

Naringenin glucuronidation in liver and intestine microsomes of humans, monkeys, rats, and mice

Author:
Isobe, Takashi, Ohkawara, Susumu, Ochi, Sadayuki, Tanaka-Kagawa, Toshiko, Jinno, Hideto, Hanioka, Nobumitsu
Source:
Food and chemical toxicology 2018 v.111 pp. 417-422
ISSN:
0278-6915
Subject:
Primates, citrus fruits, enzyme kinetics, humans, intestines, liver, liver microsomes, mice, models, monkeys, naringenin, rats, regioselectivity, toxicology, transferases
Abstract:
Naringenin, a flavanone found in citrus fruits, is mainly metabolized into glucuronide(s) by UDP-glucuronosyltransferase (UGT) enzymes in mammals. In the present study, the glucuronidation of naringenin in the liver and intestine microsomes of humans, monkeys, rats, and mice was examined. The kinetics of 7-glucuronidation in human liver and intestine microsomes followed the Michaelis-Menten model. Kinetics in mouse liver and intestine microsomes also followed the Michaelis-Menten model, whereas those in monkey and rat liver microsomes fit the biphasic model. Kinetics in monkey and rat intestine microsomes fit the Michaelis-Menten and substrate inhibition models, respectively. CLint values were mice > monkeys > rats > humans for liver microsomes, and mice > rats > monkeys > humans for intestine microsomes. In 4´-glucuronidation, activities in human liver microsomes and monkey liver and intestine microsomes were negligible or very low. Kinetics in rat and mouse liver microsomes followed the biphasic and Michaelis-Menten models, respectively. CLint values were rats > mice for liver microsomes, and rats > mice > humans for intestine microsomes. These results suggest that the metabolic abilities and regioselectivity of UGT enzymes toward naringenin in the liver and intestines generally differ between primates and rodents.
Agid:
5934764