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Chengkuizengella sediminis gen. nov. sp. nov., isolated from sediment

Cao, Wen-Rui, Guo, Ling-Yun, Du, Zong-Jun, Das, Anindita, Saren, Gaowa, Jiang, Ming-Yu, Dunlap, Christopher A., Rooney, Alejandro P., Yu, Xin-Ke, Li, Tie-Gang
International journal of systematic and evolutionary microbiology 2017 v.67 no.8 pp. 2672-2678
Gram-positive bacteria, Paenibacillus, aerobes, endospore forming bacteria, fatty acids, glycolipids, new genus, new species, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, quinones, ribosomal DNA, ribosomal RNA, sediments, sequence analysis, sodium chloride, South China Sea
A Gram-strain-positive, aerobic, motile, endospore-forming bacterium, designated strain J15A17T, was isolated from sediment of the South China Sea. The strain was oxidase-positive and catalase-negative. Optimal growth occurred at 33', pH 7.5 and in the presence of 3% (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, the strain showed closest similarity (92.81 %) with Paenibacillus puldeungensis strain CAU 9324T. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family Paenibacillaceae, with Cohnella as the closest related genus. The DNA G+C content was 37.4 mol%. The strain contained MK-7 as the sole respiratory quinone; anteiso-C15: 0 and iso-C16: 0 as the major cellular fatty acids; and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid, and four unidentified phospholipids. Phylogenetic, physiological, biochemical, and morphological differences between strain J15A17T and its closest relatives in the generas Cohnella, Fontibacillus, and Paenibacillus suggest that strain J15A17T (=KCTC 33759T=MCCC 1H00137T) represents as the type strain of a novel species in a new genus within the family Paenibacillaceae, Elvibacillus sedimins gen. nov. sp. nov.