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A premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups B, D, and E

Author:
Chen, WeiGuo, Dai, Zhenkai, Xie, Qingmei, Li, Hongxing, Zhang, Xinheng, Liu, Yang, Li, Aijun, Li, Xinjian, Yan, Yiming, Shu, Dingming, Zhang, Huanmin, Lin, Wencheng, Ma, Jingyun
Source:
Oncotarget 2017 v.8 no.62 pp. 105942
ISSN:
1949-2553
Subject:
Avian leukosis virus, alleles, binding capacity, chickens, exons, genetic resistance, glycoproteins, hosts, neoplasms, oncogenic viruses, resistance genes, selection pressure, single nucleotide polymorphism, stop codon
Abstract:
Avian leukosis virus (ALV) is an oncogenic virus causing a variety of neoplasms in chickens. The group of avian leukosis virus in chickens contains six closely related subgroups, A to E and J. The prevalence of ALVs in hosts may have imposed strong selection pressure toward resistance to ALV infection, and chickens have evolved resistance to infection by the ALVs. The tvb gene encodes Tvb receptor and determines susceptibility or resistance to the subgroups B, D, and E ALV. In this study, we characterized a novel resistant allele of the tvb receptor gene, tvbr3, which carries a single-nucleotide substitution (c.298C>T) that constitutes a premature termination codon within the fourth exon and leads to the production of a truncated tvbr3 receptor protein. As a result, we observed decreased susceptibility to infection by ALV-B, ALV-D and ALV-E both in vitro and in vivo, and decreased binding affinity of the tvbr3 receptor for the subgroup B, D, and E ALV envelope glycoproteins. Additionally, we found that the tvbr3 allele was prevalence in Chinese broiler lines. This study demonstrated that premature termination codon of the tvb receptor gene can confer genetic resistance to subgroups B, D, and E ALV in the host, and indicate that tvbr3 could potentially serve as a resistant target against ALV-B, ALV-D and ALV-E infection.
Agid:
5935357
Handle:
10113/5935357