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Effective Utilization of Carbohydrate in Corncob to Synthesize Furfuralcohol by Chemical–Enzymatic Catalysis in Toluene–Water Media
- Xue, Xin-Xia, Di, Jun-Hua, He, Yu-Cai, Wang, Bing-Qian, Ma, Cui-Luan
- Applied biochemistry and biotechnology 2018 v.185 no.1 pp. 42-54
- Escherichia coli, arabinose, catalysts, catalytic activity, cellulose, corn cobs, enzymatic hydrolysis, furfural, glucose, hemicellulose, oxalic acid, pH, synthesis, xylose, zinc
- In this study, carbohydrates (cellulose plus hemicellulose) in corncob were effectively converted furfuralcohol (FOL) via chemical–enzymatic catalysis in a one-pot manner. After corncob (2.5 g, dry weight) was pretreated with 0.5 wt% oxalic acid, the obtained corncob-derived xylose (19.8 g/L xylose) could be converted to furfural at 60.1% yield with solid acid catalyst SO₄²⁻/SnO₂-attapulgite (3.6 wt% catalyst loading) in the water–toluene (3:1, v/v) at 170 °C for 20 min. Moreover, the oxalic acid-pretreated corncob residue (1.152 g, dry weight) was enzymatically hydrolyzed to 0.902 g glucose and 0.202 g arabinose. Using the corncob-derived glucose (1.0 mM glucose/mM furfural) as cosubstrate, the furfural liquor (48.3 mM furfural) was successfully biotransformed to FOL by recombinant Escherichia coli CCZU-A13 cells harboring an NADH-dependent reductase (SsCR) in the water-toluene (4:1, v/v) under the optimum conditions (50 mM PEG-6000, 0.2 mM Zn²⁺, 0.1 g wet cells/mL, 30 °C, pH 6.5). After the bioreduction for 2 h, FAL was completely converted to FOL. The FOL yield was obtained at 0.11 g FOL/g corncob. Clearly, this one-pot synthesis strategy shows high potential application for the effective synthesis of FOL.