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Production and Biotechnological Application of Extracellular Alkalophilic Lipase from Marine Macroalga-Associated Shewanella algae to Produce Enriched C20-22 n-3 Polyunsaturated Fatty Acid Concentrate

Joseph, Dexy, Chakraborty, Kajal
Applied biochemistry and biotechnology 2018 v.185 no.1 pp. 55-71
Centrophorus, Padina, Shewanella algae, bacteria, calcium, carboxylic ester hydrolases, catalytic activity, enzyme activity, ethanol, hydrolysis, industrial applications, ions, isopropyl alcohol, liver, macroalgae, magnesium, methanol, molecular weight, oils, omega-3 fatty acids, pH, polyunsaturated fatty acids, sharks, solvents, thermal stability
An extracellular alkalophilic lipase was partially purified from heterotrophic Shewanella algae (KX 272637) associated with marine macroalgae Padina gymnospora. The enzyme possessed a molecular mass of 20 kD, and was purified 60-fold with a specific activity of 36.33 U/mg. The enzyme exhibited Vₘₐₓ and Kₘ of 1000 mM/mg/min and 157 mM, respectively, with an optimum activity at 55 °C and pH 10.0. The catalytic activity of the enzyme was improved by Ca²⁺ and Mg²⁺ ions, and the enzyme showed a good tolerance towards organic solvents, such as methanol, isopropanol, and ethanol. The purified lipase hydrolyzed the refined liver oil from leafscale gulper shark Centrophorus squamosus, yielding a total C₂₀₋₂₂ n-3 PUFA concentration of 34.99% with EPA + DHA accounting the major share (34% TFA), after 3 h of hydrolysis. This study recognized the industrial applicability of the thermostable and alkalophilic lipase from marine macroalga-associated bacterium Shewanella algae to produce enriched C₂₀₋₂₂ n-3 polyunsaturated fatty acid concentrate.