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Production and Biotechnological Application of Extracellular Alkalophilic Lipase from Marine Macroalga-Associated Shewanella algae to Produce Enriched C20-22 n-3 Polyunsaturated Fatty Acid Concentrate

Author:
Joseph, Dexy, Chakraborty, Kajal
Source:
Applied biochemistry and biotechnology 2018 v.185 no.1 pp. 55-71
ISSN:
0273-2289
Subject:
Centrophorus, Padina, Shewanella algae, bacteria, calcium, carboxylic ester hydrolases, catalytic activity, enzyme activity, ethanol, hydrolysis, industrial applications, ions, isopropyl alcohol, liver, macroalgae, magnesium, methanol, molecular weight, oils, omega-3 fatty acids, pH, polyunsaturated fatty acids, sharks, solvents, thermal stability
Abstract:
An extracellular alkalophilic lipase was partially purified from heterotrophic Shewanella algae (KX 272637) associated with marine macroalgae Padina gymnospora. The enzyme possessed a molecular mass of 20 kD, and was purified 60-fold with a specific activity of 36.33 U/mg. The enzyme exhibited Vₘₐₓ and Kₘ of 1000 mM/mg/min and 157 mM, respectively, with an optimum activity at 55 °C and pH 10.0. The catalytic activity of the enzyme was improved by Ca²⁺ and Mg²⁺ ions, and the enzyme showed a good tolerance towards organic solvents, such as methanol, isopropanol, and ethanol. The purified lipase hydrolyzed the refined liver oil from leafscale gulper shark Centrophorus squamosus, yielding a total C₂₀₋₂₂ n-3 PUFA concentration of 34.99% with EPA + DHA accounting the major share (34% TFA), after 3 h of hydrolysis. This study recognized the industrial applicability of the thermostable and alkalophilic lipase from marine macroalga-associated bacterium Shewanella algae to produce enriched C₂₀₋₂₂ n-3 polyunsaturated fatty acid concentrate.
Agid:
5936237