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Fermentative production, purification of inulinase from Aspergillus terreus MTCC 6324 and its application for hydrolysis of sucrose
- Magadum, Deepali B., Yadav, Ganapati D.
- Biocatalysis and agricultural biotechnology 2018 v.14 pp. 293-299
- Aspergillus terreus, agitation, calcium alginate, catalysts, enzyme activity, fermentation, hydrolysis, inulinase, response surface methodology, sucrose, temperature, ultrafiltration
- The production of inulinase was carried out with Aspergillus terreus MTCC 6324. To enhance the production of inulinase, fermentation media was optimized by one factor at a time and response surface methodology (RSM), which increases the activity of inulinase by 3 folds. Inulinase was partially purified with ultrafiltration method with 67.7% yield. The purified inulinase was then immobilized by entrapment method in calcium alginate beads with retention of 42% of original activity and employed for hydrolysis of sucrose. After optimization of reaction conditions such as speed of agitation, catalyst loading and temperature, the conversion of sucrose hydrolysis reaction was increased up to 91.57%.