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Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) with interferon α/γ in immediate and long-lasting protection against HP-PRRSV challenge
- Du, Yijun, Qi, Jing, Lu, Yu, Wu, Jiaqiang, Yoo, Dongwan, Liu, Xing, Zhang, Xiumei, Li, Jun, Sun, Wenbo, Cong, Xiaoyan, Shi, Jianli, Wang, Jinbao
- Virus genes 2012 v.45 no.3 pp. 474-487
- Porcine reproductive and respiratory syndrome virus, cell proliferation, immune response, interferon-gamma, interleukin-4, pork industry, recombinant vaccines, swine, vaccination, viremia
- Porcine reproductive and respiratory syndrome virus (PRRSV) has become one of the most economically important diseases to the global pork industry. Current vaccination strategies only provide a limited protective efficacy. In this study, a DNA vaccine, pVAX1©-α-γ-GP35, co-expressing GP3 and GP5 of PRRSV with interferon α/γ was constructed, and its immediate and long-lasting protection against highly pathogenic PRRSV (HP-PRRSV) challenge were examined in pigs. For immediate protection, the results showed that pVAX1©-α-γ-GP35 could provide partially protective efficacy, which was similar to the pVAX1©-α-γ (expressing interferon α/γ). For long-lasting protection, pigs inoculated with pVAX1©-α-γ-GP35 developed significantly higher PRRSV-specific antibody response, T cell proliferation, IFN-γ, and IL-4, than those vaccinated with pVAX1©-GP35 (expressing GP3 and GP5 of PRRSV). Following homologous challenge with HP-PRRSV strain SD-JN, pigs inoculated with pVAX1©-α-γ-GP35 showed almost no clinical signs, no lung lesions, and significantly lower viremia, as compared to those in pVAX1©-GP35 group. It indicated that pVAX1©-α-γ-GP35 could induce enhanced immune responses and provide both immediate and long-lasting protection against HP-PRRSV challenge in pigs. The DNA vaccine pVAX1©-α-γ-GP35 might be an attractive candidate vaccine for the prevention and control of HP-PRRSV infections.