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Determination of 3,4-dihydroxyphenylglycol, hydroxytyrosol and tyrosol purified from olive oil by-products with HPLC in animal plasma and tissues

Rodríguez-Gutiérrez, Guillermo, Wood, Sharon, Fernández-Bolaños Guzmán, Juan, Duthie, Garry G., de Roos, Baukje
Food chemistry 2011 v.126 no.4 pp. 1948-1952
brain, centrifugation, heart, high performance liquid chromatography, homogenization, kidneys, liver, muscles, olive oil, phenols, solid phase extraction, testes, white adipose tissue
A simple, fast and reliable method to quantify, simultaneously, 3,4-dihydroxyphenylglycol (DHPG), hydroxytyrosol (HT) and tyrosol (Ty) extracted and purified successfully from olive oil by-product, called alperujo, in animal plasma and tissues samples has been developed using a high-performance liquid chromatographic (HPLC) method with UV–Vis detection. Extraction of compounds is based on solid-phase extraction for plasma and homogenisation with zirconia beads and centrifugation for tissues. Calibration curves were linear for all three phenols at a relatively low concentration range (0.05–50μg/mL). This method has acceptable accuracy (91–95% in plasma and 63–100% in tissues), precision (1.11–8.26% intra-day and 0.32–9.5% inter-day) and sensitivity for detecting low concentrations of these phenols in small plasma volumes and several animal tissues such as liver, heart, kidney, muscle, testes, white adipose tissue (WAT) and brain.