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Depletion of penicillin G residues in heavy sows after intramuscular injection. Part I: Tissue residue depletion

Sara J Lupton, Weilin L Shelver, David J Newman, Steven J Larsen, D. J. Smith
Journal of agricultural and food chemistry 2014 v.62 no.30 pp. 7577-7585
benzylpenicillin, drug residues, intramuscular injection, kidneys, linear models, pharmacokinetics, procaine, skeletal muscle, sows, urine
Heavy sows (n = 126) were treated with penicillin G procaine at a 5× label dose (33 000 IU/kg) for 3 consecutive days by intramuscular (IM) injection using three patterns of drug administration. Treatments differed by injection pattern and injection volume. Sets of sows were slaughtered 5, 10, 15, 20, 25, 32, and 39 days after the last treatment; skeletal muscle, kidney, serum, and urine were collected for penicillin G analysis by LC−MS/MS. Penicillin G at withdrawal day 5 averaged 23.5 ± 10.5 and 3762 ± 1932 ng/g in muscle and kidney, respectively. After 15 days of withdrawal, muscle penicillin G residues were quantifiable in only one treated hog (3.4 ng/g) but averaged 119 ± 199 ng/g in kidneys. Using a hypothetical tolerance of 50 ng/g and a natural log−linear depletion model, the withdrawal period required for penicillin depletion to 50 ng/g was 11 days for skeletal muscle and 47 days for kidney.