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Transcriptomic and metabolomic analyses reveal that bacteria promote plant defense during infection of soybean cyst nematode in soybean

Author:
Kang, Wenshu, Zhu, Xiaofeng, Wang, Yuanyuan, Chen, Lijie, Duan, Yuxi
Source:
BMC plant biology 2018 v.18 no.1 pp. 86
ISSN:
1471-2229
Subject:
Bacillus simplex, Heterodera glycines, messenger RNA, metabolites, metabolome, metabolomics, methionine, models, palmitic acid, pathogens, plant diseases and disorders, plant growth-promoting rhizobacteria, plant parasitic nematodes, soybeans, transcriptome, transcriptomics
Abstract:
BACKGROUND: Soybean cyst nematode (SCN) is the most devastating pathogen of soybean. Our previous study showed that the plant growth-promoting rhizobacterium Bacillus simplex strain Sneb545 promotes soybean resistance to SCN. Here, we conducted a combined metabolomic and transcriptomic analysis to gain information regarding the biological mechanism of defence enhancement against SCN in Sneb545-treated soybean. To this end, we compared the transcriptome and metabolome of Sneb545-treated and non-treated soybeans under SCN infection. RESULTS: Transcriptomic analysis showed that 6792 gene transcripts were common in Sneb545-treated and non-treated soybeans. However, Sneb545-treated soybeans showed a higher concentration of various nematicidal metabolites, including 4-vinylphenol, methionine, piperine, and palmitic acid, than non-treated soybeans under SCN infection. CONCLUSIONS: Overall, our results validated and expanded the existing models regarding the co-regulation of gene expression and metabolites in plants, indicating the advantage of integrated system-oriented analysis.
Agid:
5946754