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Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats

Author:
Park, Hyun-Jung, Shim, Hyun Soo, Lee, Sunyoung, Hahm, Dae Hyun, Lee, Hyejung, Oh, Chang Taek, Han, Hae Jung, Ji, Hyi Jeong, Shim, Insop
Source:
BMC complementary and alternative medicine 2018 v.18 no.1 pp. 149
ISSN:
1472-6882
Subject:
alternative medicine, analgesic effect, antioxidant activity, antioxidants, anxiety, bioactive compounds, elevated plus-maze test, glutathione peroxidase, hippocampus, humans, hydrolysates, intravenous injection, liver function, males, menopause, nitric oxide synthase, oxidative stress, placenta, rats, staining, swimming
Abstract:
BACKGROUND: Human placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. METHODS: The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0.2 ml or 1 ml/rat) was injected intravenously 30 min before the daily stress session in male Sprague-Dawley rats exposed to repeated immobilization stress (4 h/day for 7 days). The depressive-like behaviors of all groups were measured by elevated plus maze (EPM) and forced swimming test (FST). After the behavior tests, brain samples of all groups were collected for the analysis of glutathione peroxidase (GPx) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. RESULTS: Treatment with hPH produced a significant decrease of immobility time in the FST compared to the controls. Additionally, hPH treatment elicited a slightly decreasing trend in anxiety behavior on the EPM. Furthermore, hPH increased the level of GPx protein in the hippocampus, and decreased the expression of NADPH-d in the paraventricular nucleus (PVN). CONCLUSION: This study demonstrated that hPH has anti-stress effects via the regulation of nitric oxide (NO) synthase and antioxidant activity in the brain. These results suggest that hPH may be useful in the treatment of stress-related diseases such as chronic fatigue syndrome.
Agid:
5946766