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Bleaching of biofilm-forming algae induced by UV-C treatment: a preliminary study on chlorophyll degradation and its optimization for an application on cultural heritage

Author:
Pfendler, Stéphane, Munch, Thomas, Bousta, Faisl, Alaoui-Sosse, Laurence, Aleya, Lotfi, Alaoui-Sossé, Badr
Source:
Environmental science and pollution research international 2018 v.25 no.14 pp. 14097-14105
ISSN:
0944-1344
Subject:
Chlorella, aesthetics, biodegradation, biofilm, bleaching, caves, chlorophyll, color, cultural heritage, exposure duration, irradiation, microalgae, monuments, photosynthesis, ultraviolet radiation, France
Abstract:
Green microalgae colonizing stone surfaces represent a major problem for the conservation of heritage monuments, since they lead to biodegradation and aesthetic issues. Previous studies in La Glacière show cave (France) have demonstrated that UV-C may have a strong effect on microalgae, thus leading to chlorophyll bleaching, which was increased when biofilms were maintained under VIS-light condition unlike to those maintained in the dark. To understand the physiological mechanisms underlying this response and in order to optimize in situ treatment, 30 kJ m⁻² UV-C exposure times were applied to Chlorophyta Chlorella sp. and chlorophyll degradation kinetics were then monitored. UV-C irradiation was enough to inhibit photosynthesis and to directly kill all algal cells. Results also showed that chlorophyll a was degraded faster than chlorophyll b and that 14 h were necessary for complete degradation of all the present chlorophyll. In addition, our results highlighted the importance of visible light exposition after UV-C treatment which leading to chlorophyll bleaching. Irradiated algae cultivated in the dark were still green 5 days after treatment while cultivated samples in the light lost their green color after 14 h. An efficient UV-C treatment applicable to show caves and other heritage monuments was proposed.
Agid:
5948417