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Natural resistance of Sri Lankan village chicken to Salmonella gallinarum infection

Weerasooriya, K. M. S. G., Fernando, P. S., Liyanagunawardena, N., Wijewardena, G., Wijemuni, M. I., Samarakoon, S. A. T. C.
British poultry science 2017 v.58 no.6 pp. 644-648
Salmonella Gallinarum, agglutination tests, antibodies, body weight, breeds, chickens, enzyme-linked immunosorbent assay, feed conversion, feed intake, growth performance, mortality, signs and symptoms (animals and humans), villages
1. An experiment was conducted to compare the natural resistance of an indigenous breed of local village chickens to Salmonella gallinarum with two commercial breeds: ISA Brown and ISA White layers under experimental conditions. 2. A total of 72 chickens from each of these breeds were randomly distributed to 4 pens to provide equal numbers of two replicate pens maintained as infected and control (uninfected). All chickens in infected groups were inoculated orally with 1 × 10⁸ CFU (1 ml dose) of a field isolate of S. gallinarum, at the age of 8 and 16 weeks given over 5 consecutive days. Growth performance, clinical signs, gross pathological lesions and antibody responses were measured. 3. A significantly higher mortality was observed in the brown layers compared with the white layers, and clinical signs and mortality were absent in village chickens. However, a large number of birds with gross lesions and high antibody titres were detected in village chickens, indicating that birds had the disease subclinically. Commercial breeds had a significantly higher body weight, feed intake and feed conversion efficiency. 4. There was a significantly lower proportion of positive reactors in village chickens in the whole-blood agglutination test (35%) compared to brown (100%) and white (90%) layers even after the second inoculation. Uninfected birds were negative in all groups. The indirect enzyme-linked immunosorbent assay confirmed these observations. 5. These results suggest that the indigenous breed had superior natural resistance to S. gallinarum than the commercial breeds.