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In Vitro Probiotic Properties of Lactobacillus salivarius MG242 Isolated from Human Vagina

Kang, Chang-Ho, Han, SeulHwa, Kim, YongGyeong, Paek, Nam-Soo, So, Jae-Seong
Probiotics and antimicrobial proteins 2018 v.10 no.2 pp. 343-349
Candida albicans, Lactobacillus salivarius, biological control, coculture, heat tolerance, humans, probiotics, shelf life, skim milk, storage temperature, vagina, vulvovaginal candidiasis
Vulvovaginal candidiasis (VVC) is a very common infection worldwide that is mainly caused by Candida albicans. In a previous study, we showed that Lactobacillus salivarius MG242 has anti-Gardnerella vaginalis activity. In this study, we investigated the potential of using L. salivarius MG242 for biocontrol of C. albicans. In line with the results from a spot overlay assay, MG242 inhibited the growth of C. albicans by 99.99 ± 0.01% in co-culture, suggesting that L. salivarius MG242 has the potential to be developed into a probiotic formula to treat or prevent VVC. Accelerated storage tests using dehydrated live cell powder at 50, 60, and 70 °C were performed, and the results showed that immobilization with 10% skim milk effectively increased the thermal resistance of entrapped microorganisms, resulting in sevenfold longer shelf-life than the control (in PBS). Lower storage temperatures also increased the shelf-life up to 8.31 months.