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The secondary structure of proteins in liquid, frozen, and dried egg-white samples: Effect of gamma irradiation treatment

Author:
Uygun-Sarıbay, Mine, Ergun, Ece, Kalaycı, Yakup, Köseoğlu, Turhan
Source:
International journal of food properties 2017 v.20 no.sup2 pp. 1195-1203
ISSN:
1532-2386
Subject:
Fourier transform infrared spectroscopy, egg albumen, gamma radiation, irradiation, liquids, protein secondary structure, reflectance, viscosity
Abstract:
In this study, the effect of gamma irradiation treatment on the secondary structure of the whole egg-white proteins was investigated. Liquid, frozen, and dried egg-white samples were subjected to gamma irradiation doses of 0, 1, 2, and 3 kGy. Protein secondary structures were determined by Attenuated Total Reflectance Fourier-Transform Infrared (ATR-FTIR) spectroscopy. In liquid egg-white samples, irradiation caused a conformational change from α-helix to disordered structure. Besides, a decrease in intermolecular β-sheet content was detected. On the other hand, no significant changes were observed in the secondary structure of frozen and dried egg-white samples. The significance of the results and their possible relationship to the viscosity decrease was also discussed. It was suggested that radiation-induced structural rearrangements may play a key role in the viscosity decrease.
Agid:
5953540