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Phenolics from Castanea sativa leaves and their effects on UVB-induced damage

Author:
Cerulli, Antonietta, Masullo, Milena, Mari, Angela, Balato, Anna, Filosa, Rosanna, Lembo, Serena, Napolitano, Assunta, Piacente, Sonia
Source:
Natural product research 2018 v.32 no.10 pp. 1170-1175
ISSN:
1478-6427
Subject:
Castanea sativa, antioxidant activity, chemical constituents of plants, gallic acid, humans, keratinocytes, leaves, methanol, monitoring, protected designation of origin, Italy
Abstract:
The phytochemical investigation of the methanol extract of the leaves of Castanea sativa Mill., source of the Italian PGI (Protected Geographical Indication) product ‘Marrone di Roccadaspide’ (Campania region) afforded as main compounds crenatin (1), chestanin (2), gallic acid (3), cretanin (4), 5-O-p-coumaroylquinic acid (5), p-methylgallic acid (6) and quercetin-3-O-glucoside (7). To quantify the isolated compounds a LC-ESI(QqQ)MS method working with a very sensitive and selective mass tandem experiment called Multiple Reaction Monitoring (MRM) has been developed. Moreover the antioxidant capacity by TEAC assay and the ability of compounds 1–7 to protect HaCaT human keratinocytes from UVB-induced damage has been investigated.
Agid:
5953887