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Mapping and genotypic analysis of the NK-lysin gene in chicken

Lee, Mi Ok, Yang, Ence, Morisson, Mireille, Vignal, Alain, Huang, Yong-Zhen, Cheng, Hans H, Muir, William M, Lamont, Susan J, Lillehoj, Hyun Soon, Lee, Sung Hyen, Womack, James E
Genetics, selection, evolution 2014 v.46 no.1 pp. 2625
DNA-directed DNA polymerase, adipocytes, alleles, antimicrobial peptides, antimicrobial properties, chicken breeds, chickens, chromosome mapping, chromosomes, hybrids, inbred lines, single nucleotide polymorphism
BACKGROUND: Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds. RESULTS: A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines. CONCLUSIONS: The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes, AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.