PubAg

Main content area

Dietary glutamine supplementation enhances expression of ZO-1 and occludin and promotes intestinal development in Min piglets

Author:
Zhang, Jing, Wu, Guochao, Shan, Anshan, Han, Yu, Jin, Yongcheng, Fang, Hengtong, Zhao, Yun, Shen, Jinglin, Zhou, Changhai, Li, Chunjin, Chen, Lu, Zhou, Yongfeng, Wang, Xin, Liu, Di, Yu, Hao
Source:
Acta agriculturæ Scandinavica 2017 v.67 no.1-2 pp. 15-21
ISSN:
1651-1972
Subject:
arginine, average daily gain, citrulline, dietary supplements, glutamic acid, glutamine, glutathione peroxidase, jejunum, malondialdehyde, messenger RNA, occludins, piglets, protein synthesis, superoxide dismutase, villi
Abstract:
Forty-eight Min piglets at 7 days of age were randomly divided into two groups which were fed with 0% and 0.8% glutamine, respectively. Supplementation with 0.8% l-glutamine, the average daily gain (ADG) were significantly increased, and the concentrations of glutamine, glutamate, arginine and citrulline in plasma were significantly improved (P < .05). The expression of jejunal mucosa glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) were both significantly increased, and the concentration of malonaldehyde (MDA) was significantly reduced (P < .05). Furthermore, the villus height and the villus height to crypt depth ratio were significantly increased (P < .05). Glutamine supplementation significantly increased the expression levels of occludin mRNA and protein (P < .05), while the mRNA and protein expression levels of ZO-1 were not significantly affected. These results showed that dietary supplementation with glutamine contributed to improving intestinal development and whole-body growth in Min piglets.
Agid:
5954925