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Determination of testosterone esters and nortestosterone esters in animal blood serum by LC-MS/MS Part A Chemistry, analysis, control, exposure & risk assessment

Author:
Rejtharová, Martina, Rejthar, Libor, Čačková, Katarína
Source:
Food additives & contaminants 2018 v.35 no.2 pp. 233-240
ISSN:
1944-0057
Subject:
aluminum oxide, blood serum, cattle, esters, growth promotion, meat production, monitoring, phospholipids, screening, steroid hormones, swine, testosterone
Abstract:
A sensitive and robust confirmatory method for determination of steroid esters in blood serum is essential for reliable monitoring of possible illegal use of steroid hormones as growth promoters in meat production. A previously used sample preparation methodology was improved. The procedure consists of protein precipitation and removal of phospholipids by dispersive SPE Supel™ QuE Z-Sep (Sigma-Aldrich) followed by clean-up on alumina column and LC-MS/MS measurement. The modified method has been validated according to Commission Decision 2002/657/EC. Validation parameters for determination of six testosterone esters and five nortestosterone esters in bovine and porcine blood serum are presented in this article. Decision limits for all analytes were observed in the range 10–20 pg mL⁻¹. The method described is considerably robust for bovine and porcine serum analyses and can be applied both for screening and confirmatory determination in routine residue monitoring.
Agid:
5956767